牦牛和雄性不育犏牛睾丸FABP5和FABP9基因mRNA水平及能量代谢相关酶活力的比较

doi:10.11843/j.issn.0366-6964.2015.04.008

摘要/Abstract

摘要：

本研究旨在测定牦牛表皮细胞型脂肪酸结合蛋白（FABP5）和睾丸型脂肪酸结合蛋白（FABP9）基因序列，比较其在牦牛和雄性不育犏牛睾丸中的表达，并结合睾丸中部分能量代谢相关酶的活力分析，以探索这两个基因及能量代谢与犏牛雄性不育之间的联系。试验从牦牛睾丸中提取总RNA，采用PCR方法获得了牦牛FABP5和FABP9基因的cDNA序列，编码区长度分别为408和399 bp，与普通牛相比分别有1个和6个碱基差异，后者导致FABP9基因推导的氨基酸序列存在5个氨基酸差异。实时荧光定量PCR分析显示，FABP5基因在犏牛睾丸中的表达量极显著大于牦牛，而FABP9基因表达量差异不显著。犏牛睾丸中异柠檬酸脱氢酶活力极显著高于牦牛（P<0.01），而β-羟脂酰CoA脱氢酶和乳酸脱氢酶活力与牦牛接近。犏牛睾丸中FABP5基因表达上调以及参与三羧酸循环的柠檬酸脱氢酶活力提高，可能提示雄性不育犏牛睾丸组织在脂肪酸氧化供能水平上高于成年牦牛。

Abstract:

The epidermal type fatty acid binding protein (FABP5) and testicular fatty acid binding protein (FABP9) genes of yak were sequenced，and their expressions as well as activities of some enzymes related to energy metabolism in the testes were compared between yak and sterile F1 males (male cattle × female yak)，aiming to explore the association between the two genes and energy metabolism with the sterility of F1 males.Total RNA was extracted from yak testes and the cDNA sequences of FABP5 and FABP9 genes were obtained using conventional PCR methods.The coding region of yak FABP5 and FABP9 genes were 408 and 399 bp，respectively，and showed 1 and 6 nucleotide differences compared with those of cattle，respectively，the latter caused 5 amino acid substitutions in the deduced amino acid sequences.Real-time quantitative PCR analysis showed that the expression of FABP5 gene in the sterile F1 testes was significantly greater than yak，but not for FABP9 gene.Isocitrate dehydrogenase activity in sterile F1 testes was significantly higher than yak (P<0.01)，while β-hydroxyacyl CoA dehydrogenase and lactate dehydrogenase activities were similar in the testes of yak and sterile F1 males.The up-regulated expression of FABP5 gene and the increased activity of isocitrate dehydrogenase involved in citric acid cycle in the sterile F1 testes may indicate that the level of fatty acid oxidation for energy is higher in the testes of sterile F1 males than adult yak.

中图分类号:

S823.8

付伟，黄林，刘文静，任亮，李彩霞，金素钰，林亚秋，郑玉才. 牦牛和雄性不育犏牛睾丸FABP5和FABP9基因mRNA水平及能量代谢相关酶活力的比较[J]. 畜牧兽医学报, 2015, 46(4): 561-567.

FU Wei，HUANG Lin，LIU Wen-jing，REN Liang，LI Cai-xia，JIN Su-yu，LIN Ya-qiu，ZHENG Yu-cai. Comparison of the mRNA Levels of FABP5 and FABP9 Genes and Activities of Some Enzymes Related to Energy Metabolism in the Testes of Yak and Sterile F1 Male Hybrids between Male Cattle and Female Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 561-567.

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